RNA-seq analysis of Mpwrky10 in Marchantia under nitrate and phosphate starvation, and comparative analysis with Lactuca sativa var. Redfire

To understand the role of WRKY10 transcription factor in foliar pigmentation patterns in Marchantia (liverwort) and assess the conservation of its regulatory network in Lactuca sativa var. Redfire(angiosperm). Overall design: Marchantia Polymorpha; Lactuca sativa var Redfire 7-day old gemmalings of wildtype Marchantia Tak-1 and Mpwrky10 were treated with control (2 mM phosphate and 10 mM nitrate), phosphate deficiency (0 mM phosphate) and nitrate deficiency (0 mM nitrate) for 5 days. 4-day old seedlings of Lettuce were treated with control (2 mM phosphate and 10 mM nitrate), phosphate deficiency (0 mM phosphate) and nitrate deficiency (0 mM nitrate) for 7 days.