Gene Expression Profiles of Multiple Myeloma from the sorted SP/LC and CD138+ cells

Analysis of sorted SP/LC population and a CD138+ main population cells. Results provide insight into which gene is involed in the drug resistance and tumor growth enriches for cancer stem-like cells. Keywords: multiple myeloma, cancer stem cell Side populations and CD138+ from MM patients were isolated by flow cytometry using Hoechst 33342 and CD138 antibody. The total RNA was isolated from these populations using the RNeasy RNA isolation kit (Qiagen). The RNA was then converted to cDNA and they were hybridized to the Human Genome U133Plus 2.0 GeneChip microarrays (Affymetrix). The data was normalized using the MAS5 algorithm of the Affymetrix expression console version1.1 software(Affymetrix). A comparison of transcriptional profiles was then performed in side population compared to the main population in myeloma.