Hydrolysis of fluorogenic substrates by longipain
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aFive micrograms of longipain (5 µg) were included in the standard reaction mixture containing 25 mM citric acid/sodium phosphate, pH 6.0, 5 mM DTT, 0.4 mM substrate in a final volume of 100 µl. The hydrolytic activity was monitored by spectrofluorometry at 360 nm for excitation and 465 nm for emission. bNH, not hydrolysed.
创建时间：
2013-02-21
