mRNA-sequencing for HeLa cells expressing wild-type or mutant exogenous RPB6 and shRNA against endogenous RPB6

To elucidate the biological significance of the interaction between TFIIH-p62 and RPB6, we knocked down endogenous RPB6 expression and re-expressed wild-type (WT) or mutant (F13A, F8A-F13A, or ?N20) RPB6 in HeLa cells using lentivirus vectors. While the doubling time of WT and F13A cells was close to that of parental cells (~24 h), the doubling time of F8A-F13A and ?N20 cells was over 30 h. To investigate the underlying cause of this growth defect, we performed mRNA-sequencing of WT, F13A, F8A-F13A, and ?N20 cells. Overall design: We performed mRNA-sequencing for HeLa cells expressing wild-type (WT) or mutant (F13A, F8A-F13A, or ?N20) exogenous RPB6 and shRNA against endogenous RPB6 in three biological replicates. Sequencing was performed using NextSeq 500 (Illumina).