Drosophila melanogaster S2 cells nucleosome occupancy data after simulation by heat killed Salmonella typhimurium

In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, the dynamics of nucleosomes during a genome response has not been well characterized . We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the biggest nucleosomal loss occurring at 4hr post stimulation. Overall design: Drsophila S2 cells at 0hr, 30minutes, 1hr and 4hr post heat-killed Salmonella typhimuriumstimulation