cDNA sequencing of Plasmodium falciparum field parasites 2015-2018

The objective of this study is to characterize gene expression signatures associated with in vivo artemisinin resistance phenotype and its transcriptional response to Artemisinin Combination Therapy (ACT) treatment . RNA-seq was applied to establish the global gene expression profiles for 196 and 180 isolates sampled from patients prior to and post to ACT treatment. Overall design: P. falciparum isolates were sampled from the whole blood of patients from 6 countries and 13 sites across Southeast Asia (Ramu in Bangladesh; Thabeikkyin, Pyay, Pyin Oo Lwin and Ann in Myanmar; Phusing and Khun Han in Thailand; Pailin, Pursat, Ratanakiri and Preah Vihear in Cambodia; Sekong in Laos; Bin Phuoc in Vietnam) from 2015 to 2018.