Assessment of Antibody Dilutions in Wild-Type Mice.

Host #: R = rabbit; GP = guinea pig. Peak Time: Wild-type mice were perfused at the time of expected peak of immunostaining and a series of dilutions was examined. N = number of animals tested. Results are scored as: −(no SCN cells labeled), +(relatively poor staining), ++(good staining in SCN), and +++(best staining of SCN cells compared to background). NS indicates non-specific staining (cells inside and outside the SCN were labeled with the same intensity). “n.d." indicates not determined.