Figure 4A_Competition

<b>Figure 4. The</b> <b>anti-gH antibodies bind two distinct regions of gH. </b>(a)<b> </b>Competition assays were performed with U373 gH/gL cells incubated with increasing concentrations of unlabeled antibody (5-0.1 ug/mL, x-axis) and a constant amount (0.5 ug/mL) of AF647 labeled 5C3 (top), 6E1 (middle), and 10F8 (bottom). The relative MFI of AF647-positive cells is depicted after normalization to the average MFI of labeled antibody incubated with the irrelevant influenza antibody PY102. The full set of antibodies were tested with n=2 technical triplicates and standard deviation from the mean is shown.