Fluorescence correlation spectroscopy TCSPC data with and without peak artifacts - PEX5 applied experiment

This is a dataset of Fluorescence Correlation Spectroscopy (FCS) Time-Correlated Single Photon Counting (TCSPC) data with and without peak artifacts. The provenance of the data is recorded in this file (see a rendered version here). This parent project (https://github.com/aseltmann/fluotracify) also contains examples of how to use these models and related Python code.

The following connected paper is currently under review and should be cited together with these model versions: Seltmann, A.; Carravilla, P.; Reglinski, K.; Eggeling, E.; Waithe, D. Neural Network Informed Photon Filtering Reduces Artifacts in Fluorescence Correlation Spectroscopy Data. 2023 (currently under review)

Note on the file formats and notation:

"Primary data" refers to the .ptu files, so the actual TCSPC data. "Secondary data" refers to the .pqres files, which are derived files by the proprietary PicoQuant software.

Note on sample preparation (from the Supplementary Note of the paper above):

The peak artifacts measurements were produced by 20 nM Trypanosoma brucei-PEX5 N-term fused to eGFP in solution, and the corresponding control measurements by 5 nM Homo sapiens-PEX5 N-term fused to eGFP in solution. The detailed sample preparation is described elsewhere. We prepared the samples on #1.5 coverslips mounted on Attofluor Cell Chambers (Thermo Fisher Scientific). We acquired the data on a MicroTime 200 microscope (PicoQuant) equipped with an Olympus UPlanSApo 60× 1.2NA water immersion objective lens and a HydraHarp 400 TCSPC module (PicoQuant). Excitation was achieved with a 488 nm pulsed laser (PicoQuant) with a power of 5 mW measured at the sample plane. We used a 20 nM solution of Alexa Fluor for calibrating the correction collar. One TCSPC measurement had a length of 20 s for PEX5 experiments.