Delayed administration of TAT.ARC protein attenuates focal ischemic brain injury and fosters recovery in the long-term: behavioral data of neuroscores and rota rod tests, daily temperature and body weight measures

Mice were subjected to 30 min MCAo or sham surgery and TAT.b-Gal or TAT.ARC were administered in the contralateral ventricle after 3h after the onset of ischemia and mice were observed over 28 days. DeSimoni
Neuroscore was performed at indicated time points (Fig. 8) as described (De Simoni et al., 2003; Orsini et al., 2012) with
some modifications. In brief, general health (Table 2) and specific focal
assessments (Table 3) were separately scored, analyzed and finally added to form
a summation score. Summative scores added up to  a maximum of 43 points with more points
meaning more deficits.

Rota rod was
assessed as described recently (Hoffmann et al., 2015) and
the best run out of three replicates at a given time point was used for
statistical analysis. Body temperature was measured daily as survival was documented.

Body temperature was non-invasively assessed at
the same time of the day prior to body weight measurements using subcutaneous transponders
(IPTT-300, Bio Medic Data Systems), for unambiguous identification of mice in
their home cages as described (Kort et al., 1998;
Langer and Fietz, 2014).