ATAC-seq analysis of various murine B cell subsets resulting from different immunogens.

Purpose: We aimed to link different variations of memory B cell subsets to their proliferating precursors via shared epigenetic features. Methods : Spleens were processed according to the manuscript. Cells were sorted and DNA was isolated using a protocol adapted from Corces et al. Samples were sequenced to obtain 20M 2x75 bp paired-end reads using an Illumina NextSeq 550 sequencer (Illumina, Inc, California, USA). Results: Memory cells have many DARs in comparison to FO naïve cells. However, differences between memory cells themselves can be attributed to imprinting by their respective proliferating precursors. A germinal center B cell program may be required to establish some DARs in GC-derived memory cells. Overall design: We used differentially accessible regions between DN and DPs to establish similarity between putative precursors. We used these regions to further address effects of proliferation and CD40 signals on DAR accessibility.