Expression data from in-vitro cultured mouse stress erythroid progenitors

Rapid expansion of stress erythroid progenitors is a key response to acute anemia during stress erythropoiesis. Besides the rapidly amplifying progenitors, a small population of stem-cell like erythroid progenitors undergoes limited number of cell divisions and maintains their stemness. In this study, we addressed the differences in expression profiles of regulatory genes between two stress erythroid progenitor populations that were identified by proliferation capacity and physiological status. We used microarrays to detail the gene expression profiles of stem-cell like stress erythroid progenitors and rapidly amplifying stress erythroid progenitors during stress erythropoiesis. Stem-cell like stress erythroid progenitors and rapidly amplifying stress erythroid progenitors were obtained from unfractionated mouse bone marrow culture by staining with cell division marker, PKH26, and erythroid progenitor cell surface markers and selecting by cell sorting. Total RNA was extracted from purified cell population and hybridized on Affymetrix microarrays for analysis.