Streptococcus agalactiae detection based on CRISPR/Cas12a. CRISPR/Cas12a-based assay for the rapid and high-sensitivity detection of Streptococcus agalactiae colonization in pregnant women with premature rupture of membrane

Streptococcus agalactiae or group B Streptococcus (GBS) is a leading infectious cause of neonatal morbidity and mortality. This study aims to establish a novel method, termed as the CRISPR-GBS assay, for the rapid and sensitive detection of GBS that is based on the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system.