Dataset on the changes in the gut microbiome and host metabolism in diabetic mice after transplantation of faecal microbiota from healthy young mice

Faecal microbiota transplantation (FMT) is
becoming as a promising area of interest for treating refractory diseases, but
its efficacy in diabetic cognitive decline remains unclear and the potential
mechanisms are even unknown. Herein we transplanted faecal microbiota from
healthy young mice to streptozotocin-induced type 1 diabetic
(T1D) mice with a 30-week history of diabetes and investigated their cognitive
changes using the Morris water maze
test and its possible mechanisms.
In order to examine the gut microbial changes, we used a 16S rRNA gene
sequencing approach to analyze the gut microbiome in feces of healthy control
(CON) mice, type 1 diabetic (T1D) mice, and T1D mice with faecal
microbiota transplantation from healthy young mice (FMT). Additionally, to study antibiotic
effect on host metabolism, a 1H NMR-based metabolomics approach was
applied to analyze metabolic changes in feces, colonic tissues, serum, and
hippocampus.