m6Am-seq reveals the dynamic m6Am methylation in the human transcriptome

We report m6Am-seq, based on selective in vitro demethylation and RNA immunoprecipitation. m6Am-seq directly distinguishes m6Am and 5’-UTR N6-methyladenosine (m6A). The mAm-seq is divided into a total of three steps: 1) cap-m7G RNA immunoprecipitation; 2) In vitro demethylation treatment; 3) m6A RNA immunoprecipitation. An m6Am peak could be identified by identifying the demethylase sensitivity peak between the FTO(+) and FTO(-) samples.