Expression data from wild-type single muscle fibers

The muscle stem cells (or satellite cells (SC)) reside on the muscle fibers tightly wedged between the plasma membrane of the muscle fibers and the basal lamina. The muscle fibers represent a functional niche providing paracrine signals to maintain the tissue resident stem cells. We performed microarrays to depict the global transcriptional profile of single muscle fibers at different time points We isolated purified single muscle fibers from the Extensor Digitorum Longus (EDL) muscle of Mus Musculus at different time points. The muscle fibers were isolated from postnatal pups (P3 and P7), adult mice (3-4 months old) and aged mice (24-25 months old).