The effect of Zinc defficiency on the mouse paneth cell transcriptome in TNF context

We investigated the effect of zinc depletion on the mouse intestine, and specifically on paneth cells. Mice were placed on a zinc depleted diet for 7 weeks after which they were given TNF for 3 hours and the the effects of dietary zinc shortage on paneth cells in TNF context were investigated. A zinc is an essential element for correct paneth cell function and zinc defficiency is a human and animal health problem investigating the PC transcriptom my provide valuable insights into the exact mechanism. 2 goups with 4 biological replicates each were used: one group was given the standerd chow and one group was given zinc depleted food for 7 weeks, both were injected with TNF 3 hours before sampling