Quantitative analysis of the splice variants expressed by the major hepatitis B virus genotypes

RNA-seq of Huh7 cells transfected with replication competent clones of HBV genotypes A2, B2, C2, and D3. Total RNA samples were purified using RNeasy kit (Qiagen, Hilden, Germany). The libraries were prepared using TruSeq stranded Total RNA sample preparation kit with Ribo-Zero according to the manufacturer’s protocol and sequenced using HiSeq 2500 (Illumina, CA, USA), generating 125-bp paired-end reads.