MSI data-APOL1

Apolipoprotein L1 (APOL1) high-risk genotypes G1 and G2 are responsible for the increased risk of chronic kidney disease (CKD) in the African-American population, especially in the presence of interferon gamma (IFN-γ). Here, we applied patient-derived induced pluripotent stem cells (iPSCs) to generate a kidney organoid model resembling human APOL1 nephropathy. iPSCs were generated from fibroblasts of two patients with APOL1 nephropathy homozygous for G1 or G2 risk variants (RV) and an isogenic control (G0) was created by gene correction. Kidney organoids were generated, treated with IFN-γ and incubated either with [U-13C]glucose or [U-13C]glutamine. After 4 hours of incubation, kidney organoids were quenched with liquid N2 and matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was performed for cell-type specific metabolism analysis.