Monitoring of transcript abundance in the C57BL/6 and DBA/2 ear pinnas collected at day 4 and 15 post inoculation/pi of L. amazonensis.

Ten thousand metacyclic promastigotes of L. amazonensis were inoculated into ear pinna dermis of C57BL/6 and DBA/2 mice. At days 7 and 15 pi, the ear pinnas were removed and processed for RNA isolation. Transcript abundance was determined by real-time RT-qPCR. Results are indicated as Fold Changes (FC) unexposed ears being used for calibrator for the calculation of relative expressions. One representative out of 3 independent experiments with three ears that were individually processed the means and SD being displayed.