HVN FIBRE Metadata Record - The effect of increased fibre intake on HbA1c and peripheral immune cells in diabetes

This metadata record and it's attached files make statements about the kinds of data collected as part of this research, and set out policies for governance of that data, now and in the future. Description: Increased dietary fibre and the subsequent production of short chain fatty acids (SCFA) by gut microbiota is known to influence immune cell function and metabolic activity. High fibre diets correlate to decreased inflammatory markers in T2DM, and in animal experiments dietary fibre derived SCFA have been found to reduce inflammatory markers and protect against diabetic neuropathies. Furthermore modulation of immune cell metabolism by SCFA can lead to a rebalancing away from pro-inflammatory phenotypes. We hypothesise that in addition to improving clinical indicators such as HbA1c, fibre supplementation will lead to altered peripheral immune cell metabolic and phenotypic parameters. In this study, we will conduct analyses, to allow for functional immune profiling of trial participants, to provide insights on how enhanced fibre intake in T2DM impacts immunological metabolism and function. To do this we will isolate peripheral blood mononuclear cell (PBMCs) from trial participants before and during a dietary fibre intervention. We will use high dimensional spectral flow cytometry and extracellular flux analysis to profile functional phenotypes and metabolic characteristics of individual immune populations. We will compare these, to potential changes in blood plasma cytokine levels, faecal metabolite profiles and changes in clinical readouts (eg. HbA1c).

本元数据记录及其所附文件对本次研究收集的数据种类进行了陈述，并制定了数据治理的现行及未来政策。描述如下：增高的膳食纤维摄入及其随后通过肠道微生物群产生的短链脂肪酸（SCFA）的生成，已知能够影响免疫细胞功能与代谢活动。高纤维饮食与T2DM患者中降低的炎症标记物相关，动物实验中发现，由膳食纤维衍生的SCFA能够减少炎症标记物并预防糖尿病神经病变。此外，通过SCFA调节免疫细胞代谢可能导致抗炎表型的重新平衡。我们假设，除了改善如HbA1c等临床指标外，纤维补充剂还将导致外周免疫细胞代谢和表型参数的改变。在本研究中，我们将进行数据分析，以实现对试验参与者的功能免疫谱系分析，揭示在T2DM中增加膳食纤维摄入如何影响免疫代谢和功能。为此，我们将在膳食纤维干预前后从试验参与者中分离外周血单个核细胞（PBMCs）。我们将利用高维光谱流式细胞术和细胞外流量分析来描绘单个免疫群体的功能表型和代谢特征。我们将将这些特征与血液血浆细胞因子水平、粪便代谢物谱和临床指标（例如HbA1c）的变化进行比较。