Metals such as copper (Cu) and zinc (Zn) act as essential trace elements for normal bacterial physiology but may also kill bacteria at high concentrations. Here, we employed saturated transposon mutag
spxB-encoded pyruvate oxidase is a major virulence factor of Streptococcus pneumoniae. During aerobic growth, SpxB synthesizes H2O2 and acetyl phosphate, which play roles in metabolism, signaling, and
