Transcriptional profile of B16-OVA cells with PUM1 knockout

PUM1 is a highly conserved RNA-binding protein that plays a key role in regulating mRNA stability. B16-OVA knockout PUM1 cell lines were constructed using CRISPR/CAS9 gene editing technology, and it was found that the PUM1 knockout reduce cell proliferation through indcuing G2/M phase arrest and causing cell apoptosis. From RNA-seq sequencing results, GO enrichment analysis showed that differential expression genes were mainly enriched in the negative regulation of viral process, response to interferon-beta, defense response to virus, defense response to symbionts and other pathways closely related to immunity. KEGG enrichment analysis showed that differential genes were mainly enriched in JAK-STAT signaling pathway, TNF signaling pathway, NF-kappa B signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway and NOD-like receptor signaling pathway. Overall design: RNA-seq analysis of wildtype B16-OVA cells and their knockout derivatives (KO.3, KO.11) by CRISPR/CAS9 technique