five

Newly synthesized glycoproteins during ex vivo lung perfusion

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https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=985bc599927741a6a53c5554cad731d5
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Proteins that were newly-synthesized during ex vivo lung perfusion were labeled with an azido-sugar Ac4GalNAz in the perfusate. These proteins were enriched using click chemistry to attach an alkyne-desthiobiotin group to the azido-labeled proteins, then pulled out of solution using streptavidin beads. The overall goal is to better understand the effects of warm ischemia injury in lungs destined for lung transplantation.
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2022-10-07
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