secretome study for human hepatic model during entamoeba invasion

Investigations of human parasitic diseases depend on the availability of appropriate in vivo animal models and ex vivo experimental systems, and are particularly difficult for pathogens whose exclusive natural hosts are humans, as for Entamoeba histolytica, the protozoan parasite responsible for amoebiasis. We elaborated a human hepatic in vitro model consisting of cultured liver sinusoidal endothelial cells and hepatocytes in a 3D collagen-I environment. We have characterized the model's barrier function, its chemical and structural complexity and examined E. histolytica invasion. This study deals with secretome of hepatic model under various invasion conditions.