Effects of SUMO-1 depletion on Tpr-induced senescence.

After 6 days cells transfected with Tpr or Tpr and SUMO-1, as described in Fig. 5C, were analyzed for SA-β-galactosidase staining. Forty to fifty fields were counted for each condition: the number of fields containing blue cells and the percentage of space occupied in each field by enlarged blue cells were evaluated. SABS: percentage of space occupied by blue cells in a field. Results are given as percentages of fields in the four categories for Tpr or Tpr+SUMO-1 experiments. Numbers between round brackets correspond to standard deviation. (a): only one field with blue cells was observed.