The altered σ32 phenotypes of the Tn5 insertion mutant (pftsY::Tn5) are significantly complemented by an ftsY+ plasmid.

In this and all other experiments, protein levels were determined by SDS-PAGE followed by quantitative immunoblotting. σ32 activity was determined from a chromosomal β-galactosidase reporter (calculated as a differential rate of synthesis); values presented are from ≥3 experiments. Relative S.A. of σ32 is defined as: [(σ32 activity/σ32 level) normalized to σ32 S.A. of WT cells grown at 30°C].*The ftsY+ plasmid inhibits growth of the cells by ∼30%.