ITS sequences obtained from soil samples

The data is obtained from the: 1) tillage exiperiment; 2) soil improver experiment. The samples from the tillage experiment are collected from Pärnumaa (intensive tillage, minimal tillage), Jõgevamaa (direct seeding, intensive tillage, minimal tillage), and Viljandimaa (intensive tillage, minimal tillage). The samples were collected at the beginning of the season, at the middle of the season and at the end of the season. DNA was extracted from 0.2 g of homogenised soil using the PowerMax Soil DNA Isolation kit following the manufacturer’s instructions. PCR were performed using the universal eukaryotic primers ITS9mun and ITS4ngsUni for fungal amplification following Tedersoo et al. (2021). The fungal ITS region was sequenced using the PacBio Sequel II instrument in the Norwegian Sequencing Centre at the University of Oslo. The bioinformatics were carried out using the PipeCraft platform (Anslan et al., 2017). OTU_by_sample_table.xlsx - OTU table with the final assigne taxonomy with the primary lifestyle OTU_taxonomy_10besthits.xlsx - Includes the ten best BLAST hits (including information for all the ten BLAST hits about the Align length, Mismatch, Gap openings, Query start, Query end, Target start, Query length, Target length, Query length, E-value, Bit score, and the taxonomy) Metadata_for_tillage_experiments.xlsx - Includes the metadata of the samples