The expression of regional markers in neural crest cell (NCC) aggregates treated with or without retinoic acid (RA).. The expression of regional markers in neural crest cell (NCC) aggregates treated with or without retinoic acid (RA).

To evaluate the anterior-posterior axial identity of the NCC aggregates, we prepared aggregates treated with RA to induce posterior fates as a positive control and compared gene expression patterns among the aggregates. Overall design: RNA sequencing profiles of 1231A3 iPSCs, day 5 aggregates, CD271high-sorted NCCs from day 5 aggregates, and RA-treated day 5 aggregates.