Cardiomyocyte Transcriptomes in Cardiac Development and Hypertrophy

In this study, we aim to characterize the developmental and hypertrophy-specific, or in vitro myocyte dedifferentiation-specific genes by combining the current data set of transcriptomes in fetal cardiomyocytes (ECM, E15.5), normal adult cardiomyocytes (ACM) and hypertrophic cardioymocytes (hypertrophy induced by trans-aortic constriction; TAC; 7-days post-op), with our other data set (GSE49448 -- Transcriptomic and Epigenetic Signatures of Mouse Cardiac Progenitor Cells). Differential gene expression analysis was performed to generate cell type-specific genes, including the subsets of genes important for maturity (versus dedifferentiation, immature) and cell cycle control. Transcriptomic analysis of cardiomyocytes from fetal, adult, and hypertrophic hearts. Total RNA was isolated from enriched cardiomyocytes. Illumina MouseRef-8 v2.0 Expression BeadChips were used to detect the expression of mRNA. Total RNA was isolated using Qiagen Rneasy kit. cDNA library was prepared according to Illumina BeadChip protocols for gene expression analysis. Standard microarray hybridization, scanning, and data acquisition protocols were followed according to manufacture manuals (Illumina). Gene feature data were extracted from array images using GenomeStudio software (version 2.0; Illumina).