Expression data from epethilial tom+ cells in Adenomas originated in Mex3a WT and KO animals

Mex3a labells a subpopulation of Cancer Stem cells defined by their slow proliferative behaviour. Nevertheless, the precise function of MEX3A is unknown, although it plays a role in chemoresistance. The Mex3a KO adenomas are less chemoresistant compared to their WT controls. We used microarrays to elucidate changes in gene expression in cells with the Mex3a promoter active (tomato expressing cells) Mex3a tom/flox or Tom/+ / Villin CreERT2 / APC fl/+ animals were induced with 4 consecutive shots of Tamoxifen to ensure recombination of both Mex3a and APC allele,followed by treatment with 2% DSS in drinking water for five consecutive days to enhance the number of colonic adenomas. Adenomas were collected after 2 months of the retival of DSS. Tomato positive cells (readout of Mex3a promoter activation) were purified by FACs.