Expression data from Saccharomyces cerevisiae carrying the dis3E729K mutation

Dis3 is a 3'-5' exonuclease and endonuclease that is conserved from bacteria to humans, and participates in the processing and degradation of many RNA species. DIS3 mutants suffer from impaired RNA processing, defective microtubules, growth retardation, and temperature sensitivity; however, the mechanisms by which DIS3 mutations influence cell cycle-related phenotypes remains largely unknown. Since the major impact of Dis3 mutation should be on the transcriptome, we performed an expression microarray on dis3E729K in order to elucidate the expression consequences of this allele. Strains were cultured at permissive conditions (30°C) in synthetic drop-out media selecting for the allele of interest. The wildtype control strain, as well as the mutant, both carry a URA3 marker adjacent to the DIS3 gene. Expression microarray was performed on mid-log phase culture samples. All experiments were conducted in duplicate.