ERYTHROID MITOCHONDRIAL RETENTION TRIGGERS MYELOID-DEPENDENT TYPE I INTERFERON IN HUMAN SLE

Using both in vitro-derived RBCs as well as RBCs isolated from SLE and CANDLE patients we were able to demonstrate that a defective proteasome activation during human erythropoiesis leads to the generation of pro-inflammatory RBCs that retain mitochondria. Overall design: RNA-seq of PBMC from SLE patients and RBCs derived from PBMC from healthy donors and SLE patients. RBC group definitions: Mito- RBCs group are PBMCs from SLE patients that do not carry mitochondria inside their RBCs (Red blood cells) Mito+ RBCs : Ops IgG- group are PBMCs from SLE patients that carry mitochondria inside their RBCs (Red blood cells) and they don't have opsonizing anti-RBCs IgG in their plasma Mito+ RBCs : Ops IgG+ group are PBMCs from SLE patients that carry mitochondria inside their RBCs (Red blood cells) and they have opsonizing anti-RBCs IgG in their plasma