Transcription factors Bcl11a and Bcl11b are Required for the production and differentiation of cortical projection neurons

Purpose:To assess changes in gene expression profiles of single cell from the wild type littermate controls cortices in E13.5, E15.5 and P0, and conditional knockout Bcl11a (and Bcl11b) at cortical projection neurons in the mouse cortices of the same ages. Methods: E13.5, E15.5 and P0 control and mutant cortices were carefully dissected under a clean environment. Then total RNA was isolated using an RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, quantified using NanoDrop ND-2000, and checked for RNA integrity using Agilent 2100 bioanalyzer. RNA-seq libraries were prepared according to the Illumina TruSeq protocol. Results: An average of 15 million reads per sample were obtained. RNA-seq: three mice cortical samples in each genotype at each age, totally three ages each with three different genotypes数据集用途：评估E13.5、E15.5及P0阶段野生型同窝对照小鼠大脑皮层的单细胞基因表达谱变化，以及相同发育阶段小鼠皮层内皮层投射神经元条件性敲除Bcl11a（及Bcl11b）后的基因表达谱变化。实验方法：在无菌洁净环境下精细分离E13.5、E15.5及P0阶段的对照与突变型小鼠大脑皮层。随后参照制造商操作规程，使用RNeasy Mini Kit（QIAGEN）提取总RNA；采用NanoDrop ND-2000完成RNA定量，并通过Agilent 2100生物分析仪检测RNA完整性。按照Illumina TruSeq流程构建RNA测序文库。实验结果：每个样本平均获得1500万条测序读段。RNA测序方案：每个发育阶段下每种基因型均设置3只小鼠的大脑皮层样本，共计3个发育阶段，每个阶段涵盖3种不同基因型。