Targeted sequencing (t-NGS) of frozen advanced cancers tissue was established with three panels covering 395 to 560 candidate cancer genes. Sequencing was done using the 2x150-bp paired-end technology
1Total number of mutations: EGFR, n = 1627; TP53, n = 2387. 2The number of singlets, doublets and multiplets in EGFR is 1526, 98 and 5, respectively 3The number of singlets, doublets and multiplets in