Gene expression analysis of wild type and Hedgehog pathway-activated embryonic mouse stomach

The Hedgehog signaling pathway plays important roles in gut development and homeostasis. In this study, we use Affymatrix Miroarray to analyze the gene expression profiles of wild type stomach and SmoM2-expressing stomach at E13.5. The goal of this study is to understand the transcription program regulated by Hedgehog in the developing gut. SmoM2 is a constitutively active form of Smo, whick leads to Hedgehog ligand independent cell-autonomous pathway activation. Total RNA obtained from freshly isolated E13.5 stomach tissue from wild type and NKX3.2Cre;R26-SmoM2 animals