Cell death induction activity of MtLYK3-sYFP2 mutated variants in Nicotiana leaves.

*-see [20], except for the T480A (Fig. S2), **-see [20], except for the P87S [12], K464A and T480A (this study; number of plants nodulated/number of plants tested).The designated constructs were expressed alone or co-expressed with MtNFP-mCherry construct in Nicotiana leaves. Macroscopic symptoms of cell death were scored 48 hai: only infiltrations that resulted in confluent death of (nearly) the entire infiltrated region were scored and are presented as a fraction of total infiltrations performed. # - despite the lack of pronounced macroscopic symptoms, the co-expression of MtLYK3[K464A]-sYFP2 and MtNFP-mCherry constructs resulted in increased staining with Evans blue in the entire infiltrated region.