Antipsychotic-induced epigenomic reorganization in frontal cortex samples from individuals with schizophrenia

We examined the effects of antipsychotic medications on the cell-specific epigenomics and transcriptomics in the frontal cortex of schizophrenic subjects. We performed ChIP-seq (H3K4me3 and H3K27ac) and RNA-seq on neuronal and glial nuclei of frontal cortex samples from two cohorts of postmortem schizophrenic subjects that were treated or untreated with antipsychotic medications and their controls with individually matched sex and age. In total, we tested 29 schizophrenics (15 untreated and 14 treated) and their matched control samples. Two replicates were produced on each brain sample for each assay. ChIP-seq data were generated using MOWChIP-seq (Zhu et al., Nature Protocols 14 (2019) 3366-3394.) and RNA-seq data were produced using the combination of SMART-seq2 (Picelli et al., Nature Protocols 9 (2014) 171–181.) and a Nextera XT DNA library preparation kit. The filename contains the information on the experimental group, the number of the sample within the group, the type of the brain nuclei, the epigenomic/transcriptomic data set (either H3K27ac or mRNA), and the number of replicate. For example, "SCZ_1_untreated_NeuN+_H3K4me3_R1" refers untreated schizophrenic sample #1, replicate 1 ChIP-seq data on H3K4me3. >>> Submitter declares that the raw data will be deposited in dbGaP due to patient privacy concerns. <<<