Resolving monocytes generated through TRAM deletion attenuate the pathogenesis of atherosclerosis

We performed scRNAseq analysis of murine monocytes cultured from bone marrow isolates of WT and TRAM knockout mice. WT and TRAM knockout monocytes were cultured in vitro for 5 days in the presence or absence of low dose LPS (100 pg/ml). Overall design: Monocytes isolated from C57BL/6 WT mice were cultured with low dose LPS (100 pg/ml) or PBS control for 5 days. Likewise, Tram-deficient monocytes were harvested from TRAM KO mice and cultured for 5 days with 100 pg/ml LPS or control PBS.