Unique transcriptional profiles of adult human immature neurons in healthy aging, Alzheimer's disease, and cognitive resilience

Following files submitted:1. adult_raw.RDS : Raw seurat object of the processed adult (n=24) snRNA-seq samples.2. adult_filtered.RDS : Filtered (after QC) seurat object of the processed adult (n=24) snRNA-seq samples.3. adultgc_filtered.RDS : Seurat object of adult granule cell subset after additional filtering (See Fig3A). 4. fetal_raw.RDS : Raw seurat object of the processed fetal (n=3) snRNA-seq samples.5. fetal_filtered.RDS : Filtered (after QC) seurat object of the processed fetal (n=3) snRNA-seq samples.6. neurolin.RDS : Seurat object of fetal neurogenic lineage subset (See Fig2D). 7. Functions.R : Helper functions written for running scripts.8. Fig1_script.Rmd : Script to reproduce analysis in Figure 1 and its supplementaries.9. Fig2_script.Rmd : Script to reproduce analysis in Figure 2 and its supplementaries.10. Fig3_script.Rmd : Script to reproduce analysis in Figure 3 and its supplementaries.11. Fig4_script.Rmd : Script to reproduce analysis in Figure 4 and its supplementaries.12. Fig5_script.Rmd : Script to reproduce analysis in Figure 5 and its supplementaries.13. Fig6_script.Rmd : Script to reproduce analysis in Figure 6 and its supplementaries.14. Figure 1: Isolation and snRNA-seq of the adult hippocampal niche.15. Figure 2: Isolation and snRNA-seq of the fetal hippocampal neurogenic niche.16. Figure 3: Identification of immature neurons in the adult human brain.17. Figure 4: Transcriptional features of immature neurons.18. Figure 5: In situ and in vitro validation of ImN signatures.19. Figure 6: Distinct ImN gene expression programs and intercellular interactions in AD and resilience.20. Figure S1: Integration and quality control metrics of human adult hippocampal niche snRNA-seq dataset. Related to Figure 1.21. Figure S2. Quality control and pseudotime pathway analysis of fetal snRNA-seq. Related to Figure 2.22. Figure S3. Additional quality control filtering and profiling of ImN subpopulations. Related to Figure 3.23.Figure S4. Cross-species validation of OTOF-positive granule cell population. Related to Figure 3.24. Figure S5. PCA- and UMAP-based assessment of transcriptional similarity between adult and fetal neuronal populations. Related to Figure 3.25. Figure S6. Validation of immature neuronal signatures. Related to Figure 3.26. Figure S7. Validation of immature and mature neuronal markers in publicly available spatial transcriptomic datasets across ages. Related to Figures 3-5.27. Figure S8. Transcriptional features of immature neurons across pseudotime. Related to Figure 4.28. Figure S9. Validation of immature neuronal profiles in adult human hippocampus, iPSC-derived hippocampal spheroids, and human hippocampal progenitors. Related to Figure 5.29. Figure S10. ImN gene programs across donor groups. Related to Figure 6.