RNA seq of 4-week-old SAB23KO mutants and wild-type plants soaked for 0 or 1 day, and 6 ChIP libraries sequence (two DJ and four SAB23-GFP)
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This dataset contains raw data related to the article published in the Journal of Experimental Botany, including RNAseq of 4-week-old SAB23KO mutants and wild-type plants soaked for 0 or 1 day, and 6 ChIP libraries sequences (two DJ and four SAB23-GFP)., RNA-seq:
Four-week-old SAB23KO mutant and WT plants were subjected to submergence for 0 Â or 1 d, and fully expanded leaves were collected individually for RNA-seq. Approximately 0.5 g of leaves from six seedlings of each genotype were sampled; all experiments were performed with three biological replications. The leaf samples were ground to a fine powder in liquid nitrogen, and total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA). In total, 12 RNA samples were prepared and submitted to the Beijing Genomics Institute (BGI) for sequencing.
ChIP-seq:
SAB23 was fused with GFP and transformed into DJ via Agrobacterium tumefaciens (EHA105). The transgenic lines were generated by Wuhan Biorun Biotechnology Co. Three biological replications were performed for each sample. Approximately 5 g of leaves were collected from four-week-old SAB23-GFP and DJ seedlings (as a negative control). Expression of SAB23-GFP was verified by protein gel blotting using anti-GFP antibody ..., , # RNA seq of 4-week-old SAB23KO mutants and wild-type plants soaked for 0 or 1 dayï¼and 6 ChIP libraries sequence (two DJ and four SAB23-GFP)
[https://doi.org/10.5061/dryad.k0p2ngfd3](https://doi.org/10.5061/dryad.k0p2ngfd3)
This dataset contains raw data related to the submitted article, includingRNA seq  of 4-week-old SAB23KO mutants and wild-type plants soaked for 0 or 1 dayï¼and 6 ChIP libraries sequence (two DJ and four SAB23-GFP).
6 ChIP libraries sequence (two DJ and four SAB23):
SAB23 was fused with GFP and transformed into DJ via Agrobacterium tumefaciens (EHA105). The transgenic lines were generated by Wuhan Biorun Biotechnology Co. ChIP was carried out according to Dong et al. (2019), and three biological replications were performed for each sample. Approximately 5 g of leaves were collected from four-week-old SAB23-GFP and DJ seedlings (as a negative control). Expression of SAB23-GFP was verified by protein gel blotting using an anti-GFP antibody (Abcam, AB290) at a dilutio...
创建时间:
2025-07-14



