Pancreas_sample_images
收藏doi.org2025-01-21 收录
下载链接：
http://doi.org/10.17632/nr9cyyk265.1
下载链接
链接失效反馈官方服务：
资源简介：
E14.5 embryonic mouse pancreas was sectioned at 6 µm from three embryos: embryo (emb) 2, 4 and 11
from the same litter. Two sections (300 µm apart) from each embryo were immunostained for the
proteins Carboxypeptidase 1 (CPA1), p120-catenin (p120ctn) and SRY (sex determining region Y)-box 9
(Sox9). DNA was stained with DAPI. Images were acquired as single z-plane tile scans by confocal
microscopy using a Zeiss LSM780 system and stitched with Zen software. Channel1: CPA1, Channel2:
p120ctn, Channel3: Sox9, Channel4: DAPI
E14.5胚胎小鼠胰腺经切片，厚度为6微米，分别来自同一窝的胚胎2号、4号和11号。每个胚胎选取相距300微米的两个切片进行免疫染色，以检测羧肽酶1（CPA1）、p120-连环蛋白（p120ctn）和SRY（性别决定区域Y）-盒9（Sox9）蛋白。DNA以DAPI染色。图像通过Zeiss LSM780系统采用共聚焦显微镜进行单z平面拼合扫描采集，并使用Zen软件进行拼接。通道1：CPA1，通道2：p120ctn，通道3：Sox9，通道4：DAPI。
提供机构：
Mendeley Data
