Construction of a Multihybrid Display System: Flagellar Filaments Carrying Two Foreign Adhesive Peptides
收藏PubMed Central2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC92277/
下载链接
链接失效反馈官方服务：
资源简介：
A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented ΔfliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.
提供机构：
American Society for Microbiology (ASM)
