<i>Supplemental Figures S2-S4. CA IX does not affect the cytokine, red blood cell, and platelet profiles during NH</i>_<em>4</em><i>Cl feeding and pneumonia.</i>

Wild Type (WT) and carbonic anhydrase knockout (CA IX KO) mice were fed water with 0.5% sucrose (control) or 0.28M NH₄Cl + 0.5% sucrose for 9 days. On day 7, saline or ExoY⁺ (2.5x10⁶ CFUs) was injected intratracheally and blood was collected 48 hours later for analysis of cytokines and complete blood count (CBC) with differentials. Open symbols represent female mice, and closed symbols represent male mice. Data represents means + SD. Three-way ANOVA and a Tukey’s post hoc test were used to compare groups. *Significant difference (<i>P</i> &lt; 0.05) from control.

将野生型（Wild Type, WT）与碳酸酐酶IX敲除（carbonic anhydrase IX KO, CA IX KO）小鼠分为两组，分别饲喂含0.5%蔗糖的纯水（对照组）或含0.28M氯化铵（NH₄Cl）与0.5%蔗糖的水溶液，持续饲养9天。于第7天时，经气管内对小鼠注射生理盐水或ExoY⁺菌株（2.5×10⁶菌落形成单位（colony forming units, CFUs））；48小时后采集血液样本，用于细胞因子检测及全血细胞计数（complete blood count, CBC）加白细胞分类分析。空心符号代表雌性小鼠，实心符号代表雄性小鼠。所有数据以平均值±标准差（SD）表示。采用三因素方差分析（Three-way ANOVA）与Tukey事后检验进行组间比较。*表示与对照组存在显著性差异（P < 0.05）。

搜集汇总

背景与挑战

背景概述

该数据集包含补充图S2-S4，聚焦于碳酸酐酶IX（CA IX）在NH4Cl喂养和肺炎模型中对细胞因子、红细胞和血小板谱的影响。实验使用野生型和CA IX敲除小鼠，通过血液分析评估CA IX缺失是否改变这些生理参数，结果表明CA IX不影响这些谱系的变化。数据集适用于系统生理学研究，特别是急性呼吸综合征和代谢性酸中毒相关领域。