Channel open probability parameter values for native and purified RyR1 measured in the presence and absence of 63 nM Triadin peptide and 63 nM full length Trisk 95.

The average open probability (Po) values calculated from lipid bilayer experiments are shown. Po for each channel type and presence or absence of peptide/Trisk 95 is measured from 90 s of activity at both +40 mV and −40 mV (pooled). The fold change is the Po value after trans addition of triadin peptide or full length Trisk 95, relative to Po in the absence of triadin peptide/Trisk 95. Trisk 95 was isolated from rabbit skeletal muscle and these data (in italics) have been previously published in [13] and are included here for comparison. Significant (p≤0.05) differences in Po upon addition of triadin peptide or Trisk 95 are indicated for each condition compared to its control (*).