mRNA bisulfite sequencing of pluripotent stem cells and the derived cardiomyocytes

The regulation of RNA 5-methylcytosine (m5C) modification during the differentiation process of hPSC to the derived cardiomyocytes remains unclear, and the RNA m5C have been shown to enhance innate immune response and associated with tumor progression. The purpose of this study was to provide the RNA m5Cs profiles in human pluripotent stem cells and its derived cardiomyocytes by bsRNA-seq, which provides the positions and methylation levels for future studies. Three sources of pluripotent stem cells and the derived cardiomyocytes were prepared for transcriptome-wide identification of 5-methylcytosine modifications by bsRNA-seq.