Dataset of dentin biomodifiers and odontoblast differentiation

Raw data on the effects of dentin biomodifiers on viability and differentiation into odontoblast phenotype in a dentin-human dental pulp stem cell co-culture model. The first sheet, titled "LDH Cytotoxicity," contains raw data from the evaluation of the cytotoxicity of dentin biomodifiers (0.2% chlorhexidine, 1% glutaraldehyde, 6.5% proanthocyanidin, and 600 µM myricetin). Cytotoxicity was measured by assessing the lactate dehydrogenase (LDH) released into the medium using spectrophotometry. The second sheet, titled "qPCR Odontogenic Markers," contains data on the expression of genes encoding Collagen Type I, dentin sialophosphoprotein, matrix extracellular phosphoglycoprotein, and osteopontin, evaluated by RT-qPCR. Additionally, the third sheet, titled "Alizarin Red," contains raw data from the evaluation of extracellular matrix mineralization using the alizarin red staining method, with absorbance quantified by spectrophotometry.