Gene expression in C3 and C4 Atriplex leaves, roots, and stem, and C3xC4 F1 and F3 hybrid

Elucidating the genetic control of C3 and C4 photosynthesis. Atriplex rosea (C4) and Atriplex prostrata (C3) were at maturity to compare expression between C3 and C4 in leaves, stems, and roots. Their F1 hybrid leaf was studied at maturity and will aid in identifying regulatory elements involved in C3 and C4 leaf development. Two C3 Atriplex prostrata x C4 Atriplex rosea F3 hybrids (F3003 and F3036) were sequenced at a mature leaf stage. Atriplex plants [Atriplex rosea, Atriplex prostrata, and C3 A. prostrata x C4 A. rosea F1 hybrid] were grown in biological triplicates at ~500umol photons m^-2 s^-1 under 17 hr light / 7 hr dark. Samples for RNASeq were taken from the middle of the leaf at mature leaf stage two hours after the onset of light. Stem material were taken from the most recently elongated node on the main stem. Roots were cleaned and excised from the plant. Please note that, since part of the analysis is still in progress, the 'SupplementalDataset*.txt' does not yet contain results for all samples. The dataset will be updated as soon as the results are available.