Mass spectrometry of Tse4 after final purification.

The table summarizes peptide identification results obtained from LC-MS/MS. Tse4 was identified with high confidence, achieving 100% coverage with 19 unique peptides detected. Detailed data includes peptide sequences, post-translational modifications (notably oxidation and carbamidomethylation), the ion score, molecular weight (kDa), calculated isoelectric point (pI), and retention times (RT). Peptide spectrum matches (PSMs) and charge states confirm robust detection and identification of the Tse4 protein. (XLSX)