Meiotic segregation patterns in wild-type and GAL-POL1 strains.

Tetrads were examined from AMC45 (wild-type) and AMC20 (GAL-POL1). Cells from the two strains were sporulated under identical conditions on plates containing either high or low levels of galactose. CentiMorgan distances in parentheses for the HPH�CTRP1 and TRP1�CURA3 intervals are calculated without NPDs [32]; in the HPH�CURA3 interval, distances in parentheses are calculated using only those NPDs that are the result of a 4-strand double crossovers around TRP1 (see text for details). Spore viability was 93% (wild-type, high galactose), 82% (GAL-POL1, high galactose), 90% (wild-type, low galactose), and 62% (GAL-POL1, low galactose). Sporulation efficiency was 17% (wild-type, high galactose), 19% (GAL-POL1, high galactose), 18% (wild-type, low galactose), and 17% (GAL-POL1, low galactose). PD, parental ditype; T, tetratype; NPD, nonparental ditype; cM, centiMorgans; nd, not determined because the expected value was 0.*Expected NPD tetrads if there is no interference, calculated using Stahl Lab Online Tools ��A Better Way�� (http://molbio.uoregon.edu/~fstahl/ and [35]).?Chi-square test, one degree of freedom.?Degree of interference, calculated as 1 ? (NPDobs/NPDexp).��Since there was no significant difference in the proportion of tetrad classes in cells sporulated on high and low galactose media for the wild-type cells, these data were combined.